TABLE 2.
Effect of distinct mutations in the growth kinetics of B. subtilis strains in the presence of different carbohydrates
| Carbon source | 168T+ (WT) | IQB496 (ΔaraN) | IQB611 (ΔaraNPQ) | IQB 608 (araE::kan) | IQB610 (ΔaraN araE::kan) | IQB495 (ΔmsmX::cat) | IQB609 (ΔmsmX::cat araE::kan) |
|---|---|---|---|---|---|---|---|
| Glucose | 55.5 ± 1.3 | 53.4 ± 1.0 | 52.5 ± 0.8 | 57.3 ± 0.3 | 58.6 ± 1.5 | 57.7 ± 2.7 | 54.1 ± 1.1 |
| Arabinose | 67.0 ± 0.2 | 66.6 ± 0.9 | 63.7 ± 2.9 | NG | NG | 68.2 ± 2.3 | NG |
| Arabinobiose | 112.4 ± 10.8 | 263.8 ± 37.2 | 258.2 ± 46.1 | 178.6 ± 19.9 | NG | 286.7 ± 33.1 | NG |
| Arabinotriose | 98.2 ± 10.0 | NG | NG | - | - | NG | - |
| Arabinotetraose | 92.4 ± 4.5 | NG | NG | - | - | NG | - |
| Arabinan (sugar beet) | 149.0 ± 6.8 | 177.0 ± 21.6 | 188.8 ± 15.3 | - | - | NG | - |
| Debranched arabinan | 84.0 ± 4.6 | 347.6 ± 23.7 | 330.6 ± 30.8 | - | - | 332.5 ± 6.8 | - |
| Maltoriose | 77.7 ± 2.3 | - | - | - | - | 83.1 ± 2.1 | - |
| Maltotetraose | 76.1 ± 2.2 | - | - | - | - | 125.8 ± 4.5 | - |
| Maltopentaose | 75.0 ± 1.2 | - | - | - | - | 105.4 ± 4.2 | - |
The B. subtilis wild-type (WT) and different mutant strains were grown in minimal CSK liquid medium in the presence of the following sugars (0.1%, wt/vol): glucose, arabinose, α-1,5-arabinobiose, α-1,5-arabinotriose, α-1,5-arabinotetraose, arabinan (sugar beet), debranched arabinan, maltotriose, maltotetraose, and maltopentaose. The kinetic growth parameters were determined, and the results correspond to the averages from three independent experiments and respective standard deviations. NG, no growth; -, not determined.