FIG. 5.

(A) SDS-PAGE of T. neutrophilus proteins eluted from a DNA affinity chromatography column using scr-4hbl and 4hbd-frd promoter/operator probes. Cells were grown on CO₂ or in the presence of acetate. The proteins in lanes 1 and 3 were eluted with 0.3 M NaCl, and those in lanes 2 and 4 were eluted with 1.0 M NaCl. Assignments for proteins a to e are given in the text. (B) Electrophoretic mobility shift assay. (Left) SDS-PAGE of the recombinant Tneu_0751 protein. (Center and right) EMSAs using promoter DNA or control DNA with protein at the indicated molar excesses. (C) SDS-PAGE of recombinant Alba protein and EMSAs as described for panel B. Because of a second start codon inside the gene, a shortened Alba protein was also overexpressed.