TABLE 2.
Comparative proteome analysis (soluble proteins) of cells grown autotrophically and cells grown in the presence of acetate
| Gene | Enzymea | Mass (kDa) | pI | Spot no. | Coverage (%) | Scoreb |
|---|---|---|---|---|---|---|
| Genes encoding enzymes participating in the dicarboxylate/hydroxybutyrate cycle | ||||||
| Tneu_1795 | Pyruvate:ferredoxin oxidoreductase TPP-binding subunit | 36.8 | 8.4 | 1 | 52 | 53 |
| Tneu_1204 | Pyruvate-water dikinase (PEP synthase) | 89.4 | 5.8 | 2 | 55 | 301 |
| Tneu_0418 | PEP carboxylase | 51.4 | 6.4 | 3 | 65 | 209 |
| Tneu_1509 | Malate dehydrogenase (NAD+) | 33.0 | 7.0 | 4 | 70 | 185 |
| Tneu_1464 | Succinyl-CoA synthetase, alpha subunit | 30.1 | 8.9 | 5 | 81 | 184 |
| Tneu_1463 | Succinyl-CoA synthetase, beta subunit | 41.6 | 6.0 | 6 | 58 | 164 |
| Tneu_0420 | 4-Hydroxybutyrate-CoA ligase (AMP forming) | 71.2 | 6.8 | 7 | 58 | 263 |
| Tneu_0422 | 4-Hydroxybutyryl-CoA dehydratase | 55.4 | 7.8 | 8 | 57 | 198 |
| Tneu_0541 | Crotonyl-CoA hydratase/3-hydroxybutyryl-CoA dehydrogenase | 72.3 | 6.3 | 9 | 66 | 350 |
| Genes encoding other proteins | ||||||
| Tneu_0415 | CBS-containing domain protein | 15.3 | 6.2 | 10 | 81 | 131 |
| Tneu_1383 | Putative RNA-associated protein | 25.9 | 9.0 | 11 | 77 | 250 |
| Tneu_0708 | Putative 3-hexulose-6-phosphate synthase | 23.7 | 6.0 | 12 | 77 | 173 |
a
Enzymes involved in the CO2 assimilation of T. neutrophilus, which were induced exclusively under autotrophic growth conditions, as well as some other autotrophic-growth-induced proteins, are listed. For proteome analysis, see Fig. S1 in the supplemental material. TPP, thiamine pyrophosphate.
b
Calculated as −10 × log(P), where P is the probability that the observed match is a random event. Protein scores greater than 45 are considered significant (P < 0.05).