TABLE 5.
Differential expression of rsmAYZ in planktonic and biofilm cells
| Growth condition(s) and strains | Fold change of transcripta |
||
|---|---|---|---|
| rsmA | rsmY | rsmZ | |
| Planktonic | |||
| ΔbfiS strain vs PAO1 | 1.10 (±0.36) | 1.39 (±0.21) | 1.30 (±0.27) |
| Biofilm vs planktonic cells | |||
| PAO1 (24 h biofilm) | 0.27 (±0.08) | 0.22 (±0.05) | 0.10 (±0.04) |
| PAO1 (144 h biofilm) | 0.28 (±0.02) | 0.61 (±0.25) | 0.23 (±0.08) |
| ΔbfiS strain (24 h biofilm) | 0.21 (±0.20) | 0.11 (±0.09) | 0.05 (±0.01) |
| ΔbfiS strain (144 h biofilm) | 1.12 (±0.22) | 1.67 (±0.34) | 3.51 (±1.04) |
| Biofilm | |||
| PAO1/pJN-cafA vs PAO1/pJN105 | 0.89 (±0.09) | 1.24 (±0.28) | 0.30 (±0.07) |
a
Relative ratio of expression, standardized to the expression levels of the housekeeping gene mreB, as revealed by qRT-PCT analysis. Standard deviations are given in parenthesis.