FIG. 3.

The ydzH-ydfR intergenic gene and its paralog are expressed in the mother cell. (A) Fluorescent micrographs of cells expressing gfp fused in frame to the 3′ terminus of the small ORF in the ydzH-ydfR intergenic region (IGR HR1-GFP) and to the 3′ terminus of its paralog ypzD (YpzD-GRP). The cells were collected 2 h after induction of sporulation by resuspension and stained with a fluorescent membrane dye. The merged images (GFP, green; membrane, blue) show representative cells expressing GFP in the mother cell. (B) Alignment of inferred amino acid sequences for the newly identified ORF in the ydzH-ydfR region (IGR HR1) and YpzD. The proteins have 47% identical and 67% similar amino acids, as indicated by the consensus amino acid and a plus sign, respectively.