TABLE 1.
Oligodeoxynucleotide primers used in this worka
| Primer name | Sequence (5′→3′) |
|---|---|
| alr0819-7120-1 | CATAGAAGAATCAGCATGGGAAG |
| alr0819-7120-2 (R1) | CGGTAGGCGGTTATCAATTGCTTC |
| alr0819-7120-3 | ATCGATGGTGTACAATATATGATCC |
| alr0819-7120-4 | GATATCCAGTCCGTTTAGCTTCTGC |
| alr0819-7120-5 | GTGTACAATATATGATCCTTGC |
| alr0819-7120-6 | ATCTACGATATCCAGTCCGTTTAGCTTCTGC |
| alr0819-7120-7 | GGACTGGATATCGTAGATGCTTCTATATCTCG |
| alr0819-7120-8 | CTTCAGCCAACCGGATATATTGCC |
| alr0819-7120-9 (F7) | GTATCATGGTTGTGGTGGGC |
| alr1521-7120-1 | GTACTGTAGCTGCTCAAGATCCAG |
| alr1521-7120-2 (R2) | GATGCGTTGAGATTCTTCCTCG |
| alr1521-7120-3 | ATCGATTGCTCAGAAGGCAGGAGTCAGCG |
| alr1521-7120-4 | GATATCATTAATTGGAGGGGTTTTC |
| alr1521-7120-5 | TGCTCAGAAGGCAGGAGTCAGCG |
| alr1521-7120-6 | GGGCCGGATATCATTAATTGGAGGGGT |
| alr1521-7120-7 | ATTAATGATATCCGGCCCTGGTTGTAGTC |
| alr1521-7120-8 | CTTATCAAGAGAGTATCCCACAAGC |
| alr1521-7120-9 (F8) | GGGACACGGGGTATCGGTATAG |
| CS.3-2 | GTTACCCGAGAGCTTGGC |
| CK.3-3′ | GGTGCCCTTAAACGCCTGGTG |
| GFP-4 | CAAGAATTGGGACAACTCC |
| NH1 | GTACTGCAAGGGGCGTGTGGC |
| NH4 | CCTATTGGTAGCTTCTGCGGG |
| M13-Reverse | GAGGAAACAGCTATGAC |
| M13-Universal | GTAAAACGACGGCCAGT |
a
Introduced restriction enzyme cutting sites are underlined.