FIG. 2.

Truncational analysis to delineate the arabinoxylan-binding region in FSUAXH1. (Left panels) Schematic representations of the truncated constructs of FSUAXH1. The primers used for the amplification of the genes are shown above the schematic for the protein constructs, and the primer nucleotide sequences are presented in Table 1. The arrowheads display the 5′ to 3′ direction of oligonucleotides. (Right panels) Qualitative binding analysis results for FSUAXH1 and its truncational derivatives by affinity gel electrophoresis. Two micrograms of each protein was resolved on a nondenaturing 5% polyacrylamide gel containing 0% (control) or 0.1% (wt/vol) arabinoxylan and stained with Coomassie brilliant blue G-250. The reason for the slower migration of TM2 (in the control) is currently unknown.