TABLE 1.
Primers used in this study
| Primer | Sequence | Expt type |
|---|---|---|
| F1 | 5′-GACGACGACAAGATGGCCGTTAAGGTCAATAACCCG-3′a | Cloning |
| F2 | 5′-GACGACGACAAGATGGGCTACGGCATGGTCACGAGTGAC-3′a | Cloning |
| F2A | 5′-GACGACGACAAGATGGGCAAGTGGTATGCCCTCCTGTTCC-3′a | Cloning |
| F2B | 5′-GACGACGACAAGATGGGTCCGGTTGGCCGTATGTCG-3′a | Cloning |
| F2C | 5′-GACGACGACAAGATGGGATCCAAGGCTCCCTCGACG-3′a | Cloning |
| F3 | 5′-GACGACGACAAGATG GGCGAAAACTGCCCTGCAAATGC-3′a | Cloning |
| R1 | 5′-GAGGAGAAGCCCGGTTACTTTGTTACGGATAGGCGGTGGG-3′a | Cloning |
| R2 | 5′-GAGGAGAAGCCCGGTTAAGCGTTTGCGCCCTTCACGAACG-3′a | Cloning |
| R3 | 5′-GAGGAGAAGCCCGGTTAGCCGTCGAGATAAATTTCATCGTTCAC-3′a | Cloning |
| R4 | 5′-GAGGAGAAGCCCGGTTAGCCCGGGAGCGGAGATTCCGG-3′a | Cloning |
| R5 | 5′-GAGGAGAAGCCCGGTTAGCCAACAATGGGATCCGGATCCG-3′a | Cloning |
| W337A | 5′-GGCGAACTTGCTCTCGAAGCGCAGTTCAACCATAACCCT-3′b | Mutagenesis |
| W348A | 5′-CCATAACCCTGATAACAAAAACGCGAGCTTGTCTGCAAATCCGGG-3′b | Mutagenesis |
| Y465A | 5′-CCGTGGTACTGCATATTTCGCTTACAGCACCGATGGTAGC-3′b | Mutagenesis |
| Y466A | 5′-GGTACTGCATATTTCTATGCCAGCACCGATGGTAGCTCTTGG-3′b | Mutagenesis |
| W473A | 5′-GCACCGATGGTAGCTCTGCGAAAAAGATTGGCAACGATG-3′b | Mutagenesis |
| Y484A | 5′-GGCAACGATGTGAAGCTCAATGCTGACCTCCACATGTTCGTG-3′b | Mutagenesis |
| Y507A | 5′-CCAAGCAGGCGGGCGGCGCTGCAGACTTCGACTGGTTC-3′b | Mutagenesis |
| D148A | 5′-GCAGCTCCCGTTCTACCATGCCCCTTCTTTGTTCTTTGATGAC-3′b | Mutagenesis |
a
Nucleotides incorporated for exonuclease digestion are underlined.
b
Nucleotides corresponding to the substituted amino acids in the mutagenesis experiments are underlined.