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Kinetic parameters of FSUAXH1 proteinsa
| FSUAXH1 protein form | kcat (s−1) | Km (mg/ml) | kcat/Km [s−1 (mg/ml)−1] |
|---|---|---|---|
| WT | 240 ± 23 | 4.1 ± 1.0 | 58 ± 16 |
| TM1 | 360 ± 40 | 5.4 ± 1.6 | 67 ± 21 |
| TM2 | 310 ± 21 | 3.4 ± 0.70 | 89 ± 19 |
| TM2-Y484A | 630 ± 120 | 24 ± 7.1 | 27 ± 10 |
| TM2-D148A | NDb | ||
| TM2-D148A/Y484A | ND | ||
| TM3 | ND |
Data are means ± standard errors based on the nonlinear regression model.
ND, not detected; activity was below the detection limit of the assay.
