FIG. 5.

Analysis of A9, BL3, and NF5 protein composition. (A) Identification of virus structural proteins by peptide fingerprinting. Protein molecular mass marker masses (in kilodaltons) and identified gene products are indicated. Abbreviations: Tsh, tail sheath protein; Cps, major capsid protein; Tmp, tape measure protein; gp, gene product; -L, longer protein variants produced by programmed translational frameshifting. (B) Proposed locations of frameshifts in structural protein genes of Brochothrix bacteriophages. The type of frameshift, location within the genome, and slippery sequences (lines) are indicated. The putative frameshifts in phages NF5 and A9 were experimentally verified by peptide mass fingerprinting (see Table S6 in the supplemental material).