FIG. 2.

RNA degradosome of P. haloplanktis. SDS-PAGE stained with Sypro orange. Whole-cell extracts were prepared from P. haloplanktis conjugated with the empty shuttle vector (pIB3) or the vector encoding FLAG-tagged Ph-RNase E (pSAB17). After absorption to the anti-FLAG agarose beads and being washed, Ph-RNase E was eluted with FLAG peptide. Lane 1, pIB3 (background control); lane 2, pSAB17 (FLAG-tagged Ph-RNase E). M, molecular mass markers. The proteins shown in lane 2 were identified by mass spectrometry. The asterisks indicate proteolytic products of Ph-RNase E.