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. 2010 Aug 20;192(20):5413–5423. doi: 10.1128/JB.00592-10

FIG. 4.

FIG. 4.

Complementation of KSL2000, expression of Ph-RNase E at 30 and 15°C, and growth of the MC1061 rne131 strain at 15°C. (A) Complementation of the KSL2000 strain (the chromosomal gene encoding Ec-RNase E was disrupted) by a plasmid expressing FLAG-tagged Ph-RNase E (pSAB18). Serial dilutions (10-fold) were spotted on LB plates and grown at the indicated temperatures for times ranging from 1 day (37 and 42°C) to 10 days (15°C). (B) SDS-PAGE stained with Sypro orange and Western blotting using anti-FLAG antibodies. Lane 1, KSL2000 complemented with pAM-rne (Ec-RNase E without FLAG); lane 2, pVK200 (Ec-RNase E with FLAG); and lane 3, pSAB18 (Ph-RNase E with FLAG). M, molecular mass markers. (C) Growth of MC1061 (wild type [wt], AC21) and the rne131 derivative (AC27) at 30 and 15°C (10-fold serial dilutions).