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. 2010 Aug 20;192(20):5413–5423. doi: 10.1128/JB.00592-10

FIG. 5.

FIG. 5.

Ph-RNase E expressed in E. coli interacts with Ec-RhlB. SDS-PAGE stained with Sypro orange. (A) Immunopurifications were performed as shown in Fig. 2. Lane 1, KSL2000 complemented with pAM-rne (Ec-RNase E without FLAG); lane 2, pVK200 (Ec-RNase E with FLAG); and lane 3, pSAB18 (Ph-RNase E with FLAG). M, molecular mass markers. The asterisks indicate subunits of pyruvate dehydrogenase, which interacts adventitiously with the FLAG antibody. (B) The experiment shown in panel A was repeated using a strain (SVK446) in which the gene encoding RhlB was disrupted.