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Effects of MurC and l-alanine on cell length. Cells of the triple mutant (A to E) were grown overnight in glucose minimal medium and subcultured into NGCAA for 6 h with no l-alanine (A) or with l-alanine added at 50 (B), 100 (C), 200 (D), and 500 (E) μg/ml. The triple mutant carrying plasmid JW0089 (pmurC) (F to H) grown in the same way was subcultured into NGCAA for 6 h with 100 μM IPTG and l-alanine (50 μg/ml) (F), with 100 μM IPTG alone (G), and with no further additions (H).
