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Effects of MurC and l-alanine on cell appearance. Cells of the triple mutant (A) and the triple mutant carrying plasmid JW0089 (pmurC) (B) were grown overnight in glucose minimal medium and subcultured into NGCAA without l-alanine (i) or with l-alanine added at 50 (ii), 100 (iii), and 500 (iv) μg/ml for 6 h. The images are taken from one experiment and representative of two.
