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Effect of control of the Pmra promoter by IPTG. Cells of the triple mutant with the Hara promoter (strain MEW999H) were grown overnight in glucose minimal medium and subcultured into NGCAA with 1 mM IPTG (C and D) or without IPTG (B). The pictures were taken at 0 (A), 3 (B and C), and 9 (D) hours.
