TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristic(s) | Source and/or reference |
|---|---|---|
| Strains | ||
| Cu1008 | E. coli K-12 ilvA | L. S. Williams |
| MEW1 | Cu1008 Δlac | 24 |
| MEW999 | MEW1 ΔsdaA ΔsdaB ΔtdcG | 32 |
| JE7968 | Pmra::Plac; IPTG-dependent strain; Cmr | 16 |
| MEW999H | MEW999 derivative; Pmra::Plac; IPTG-dependent strain; Cmr | This study |
| Plasmids | ||
| JW0089 | pCA24N carrying murC | ASKA library (14) |
| JW0087 | pCA24N carrying ftsW | ASKA library (14) |
| JW0086 | pCA24N carrying murD | ASKA library (14) |
| JW0083 | pCA24N carrying murE | ASKA library (14) |
| JW0084 | pCA24N carrying murF | ASKA library (14) |
| JW0094 | pCA24N carrying lpxC | ASKA library (14) |
| JW0093 | pCA24N carrying ftsZ | ASKA library (14) |
| JW0081 | pCA24N carrying ftsL | ASKA library (14) |
| JW0091 | pCA24N carrying ftsQ | ASKA library (14) |
| JW0082 | pCA24N carrying ftsI | ASKA library (14) |
| JW0372 | pCA24N carrying ddlA | ASKA library (14) |
| JW0090 | pCA24N carrying ddlB | ASKA library (14) |
| psamT | pLtet plasmid carrying the rickettsial SAM transporter from pSMART | 26 |
| pGS146 | Expression plasmid carrying the gcvTHP operon | 23 |