TABLE 3.
Expt | Time (h) | Addition of SAM (2 mM) | No. of cells measured | Length (μm) |
% of cells of the following length (μm) |
|||||
---|---|---|---|---|---|---|---|---|---|---|
Avg | Minimum | Maximum | <5 | 5 to 10 | 10 to 50 | >50 | ||||
1 | 6 | + | 54 | 23.6 | 7.3 | 58.5 | 0 | 6 | 87 | 7 |
2 | 8 | + | 53 | 46 | 3.6 | 143.5 | 4 | 10 | 43 | 43 |
3 | 10 | + | 132 | 34 | 4.6 | 179.8 | 2 | 17 | 60 | 22 |
4 | 6 | − | 23 | 10.0 | 4.4 | 17.2 | 13 | 39 | 48 | 0 |
5 | 8 | − | 30 | 25.4 | 6.6 | 57.5 | 0 | 7 | 80 | 13 |
6 | 10 | − | 95 | 28.6 | 4.6 | 80.6 | 1 | 14 | 68 | 18 |
Cells of the triple mutant carrying plasmid psamT were grown with 2 mM S-adenosylmethionine (SAM) (experiments 1 to 3) and without SAM (experiments 4 to 6) in NGCAA and photographed after 6, 8, or 10 h as noted. Photographed cells were measured, and average, maximum, and minimum lengths were determined.