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Sequence alignment of VRSA-9, VRSA-6, and VRSA-7 Ddls. Dots indicate identical amino acids; active-site residues are highlighted in dark gray; underlined residues are conserved in all d-Ala:d-X ligases; amino acid substitutions in VRSA-7 and VRSA-9 Ddls are indicated in bold. Numbering above the sequences indicates amino acids interacting with ligands (1, d-Ala1; 2, d-Ala2; 3, ATP; 4, Mg2+).
