TABLE 3.
Kinetic parameters for d-Ala-d-Ala synthesis of VRSA-9 Ddl in comparison with those of VRSA-6 and VRSA-7 Ddls
| Ddl | Km1a (mM) | Km2b (mM) | kcat(min−1) | kcat/Km2(min−1/mM) | Relative kcat/Km2 | ATP Km (mM) |
|---|---|---|---|---|---|---|
| VRSA-6c | 1.2 | 17 | 1,925 | 113 | 1 | 0.70 |
| VRSA-7c | NDd | 76.5 | 7.6 | 0.1 | 0.9 × 10−3 | 0.86 |
| VRSA-9 | 2.0 | 240e | 126f | 0.52 | 4.6 × 10−3 | 4.0g |
a
Km at subsite 1.
b
Km at subsite 2.
c
Data from reference 23.
d
ND, not detectable.
e
Highest substrate concentration tested.
f
Activity measured with 15 μg of enzyme (1.3 μM).
g
Apparent Km determined with 80 mM d-Ala.