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. 2010 Jul 23;192(20):5289–5303. doi: 10.1128/JB.00460-10

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FIG. 7. — Frameshifting resulting from indel mutations. (A) IS895s. The partial sequence and the bars that represent IS(alr0552, alr0553) also represent IS(alr1853, alr1854), IS(all1972, all1971), IS(all2067, all2066), IS(alr2773, alr2774), and IS(all4868, all4867). The poly(A) region, positions 594 through 603, determines how long an ORF continues. The ORFs end (*) at position 658 if all 10 As remain, at position “611” if 1 A is removed, and (not shown) 9 bp from the R end of the IS if 2 As are removed. (B) IS891-related cluster 2. The partial sequence and the bar that represent IS(all4465) also represent IS(all7325). Due to a deleted nucleotide at position ca. 392, All0315 terminates at amino acid position 136 (*), whereas All4465 continues further. Lowercase black letters in the bottom parts of panel B represent the hypothetical amino acid sequence in the absence of the single-nucleotide deletion. (C) IS892, aka IS(all7268), and related ISs. The partial sequence and the bar that represent IS(all7268) also represent IS(all7323) and IS(alr8510). With a single base pair absent from the poly(A) region of positions 850 through 859, the ORF continues to 18 bp before the R end of the IS (not shown). The partial sequence and the bars that represent IS(all7005, all7004) also represent IS(all7106, all7105), IS(all7112, all7111), IS(all7178, all7177), IS(all7303, all7302), and IS(all7376, all7375). With no deletions in the aforementioned poly(A) region, the first ORF ends at position 864. With the G lost at position 1053 within the A-rich region of positions 1047 to 1055 (bottom part of panel C), asr8501 ends at position 1076. (D) ISAzo13-family, cluster 2. Deletion of a T at position 696 of N. punctiforme ISs (represented by np) adjacent to a poly(A) region causes truncation of the np ORFs at position 712. However, as in panels A to C, ribosomal frameshifting in the immediately succeeding poly(A) sequence may allow continued translation of the sequence.