FIG. 8.

Palindromes at the ends of IRs of copies of IS895-related ISs. (A) If an IS ends in, or is flanked by, a palindrome (here, TA or TATA, and underlined), that palindrome can form part of the IRs (interpretations 1 to 4), DRs (3 or 5), or both (3), resulting, as shown in panel B, in different frameshifts when the IS and one copy of a DR are computationally removed. Further information is required to determine which is correct in a particular case. (C to E) Evidence that IS(all7564, all7563) in cluster 5 (C and D) and IS(alr1726, alr1727) in cluster 1 (E) generate a TA direct repeat (capitalized and underlined) upon insertion, by comparison with homologous nucleotide (C and E) and protein-encoding (D) regions in A. variabilis (Subject). (F) That interpretation (A3, B3) is here shown applied to all members of cluster 1.