FIG. 7.

AmrZ DNA-binding activity is required for optimum virulence. (A) A total of 10⁸ PAO1 or WFPA513 (R22A AmrZ) bacteria were used to intranasally inoculate 6-week-old C57BL/6 mice. At 4 and 12 hpi, lungs were aseptically harvested, and the bacteria were enumerated. Each symbol indicates an individual mouse (n = 15 for each group per time point). Gray bars indicate averages along with standard deviations (**, P = 0.003). (B) Mice (n = 15) were assessed for bacterial colonization (>10⁴ bacteria recovered from the lungs), bacteremia (bacteria recovered from the blood), or death (requiring euthanasia due to severe disease symptoms) at 12 hpi.