TABLE 2.
AmrZ residues Lys18, Val20, and Arg22 are absolutely required for activation of algD transcriptiona
| Strain | Genotype | AmrZ expressed | Avg β-galactosidase activity (Miller units) ± SD | % of wild-type activity |
|---|---|---|---|---|
| FRD2606 | mucA22 attB::algD-lacZ | Wild type | 10,011 ± 44 | 100 |
| FRD2526 | mucA22 amrZ::Ωtet attB::algD-lacZ | 5 ± 0.9 | 0.05 | |
| FRD2534 | mucA22 amrZ37 attB::algD-lacZ | AmrZ R14A | 285 ± 5 | 3 |
| FRD2517 | mucA22 amrZ17 attB::algD-lacZ | AmrZ K18A | 0 ± 0.9 | 0 |
| FRD2519 | mucA22 amrZ18 attB::algD-lacZ | AmrZ V20A | 65 ± 2 | 0.6 |
| FRD2521 | mucA22 amrZ19 attB::algD-lacZ | AmrZ R22A | 0 ± 0.6 | 0 |
a
Single-copy algD-lacZ transcriptional fusions were placed at the neutral attB site in isogenic amrZ+ (FRD2606), AmrZ R14A (FRD2531), AmrZ K18A (FRD2517), AmrZ V20A (FRD2519), and AmrZ R22A (FRD2521) strains of P. aeruginosa. All strains were engineered in an FRD1 (mucA22) background. β-Galactosidase activity was recorded in Miller units, and results are average data from three separate experiments. Percentages of wild-type values are also listed for comparison purposes.