FIG. 5.

(A) Effects of knocking down scaffold proteins, JIP1 (left) and KSR1 (right), on the SRE-dependent transcriptional response to EGF in HeLa cells. ***, P < 0.001. The error bars indicate standard deviations. (B) Effects of siVRK2 and siKSR1 on the SRE-dependent transcriptional response to EGF in HeLa cells. **, P < 0.005; n.s., not significant. (C) Effect of VRK2A in response to EGF after knockdown of KSR1 in MCF7 cells. *, P < 0.01. (D) Effects of KSR1 and VRK2 knockdown on phospho-Erk levels in MDA-BB-231 cells in the presence of EGF. The effect on the activation of the SRE promoter is also shown (right). The change in luciferase activity resulting from activation of the SRE-luc reporter coincides with the result for phospho-ERK (left).