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. 2010 Aug 2;30(19):4604–4615. doi: 10.1128/MCB.00197-10

FIG. 4.

FIG. 4.

Impaired v-Src-mediated tyrosine phosphorylation in KO-NY72 cells. WT-NY72 and KO-NY72 cells were shifted to the permissive temperature and collected at the indicated times. Cell lysates were prepared and subjected to immunoblotting with an antiphosphotyrosine antibody. The intensity of the phosphotyrosine signal in the lysates of WT-NY72 and KO-NY72 cells at 4 h after the temperature shift was determined by densitometry and is shown below.