FIG. 5.

Impaired transformation in vitro of KO-NY72 cells. (A) Soft agar colony formation. WT-SV40, KO-SV40, WT-NY72, and KO-NY72 cells were cultured in 0.36% agarose for 2 weeks until colonies of WT-NY72 cells became visible. Colonies were stained with MTT, and the number of colonies in each dish was determined. The experiment was performed in triplicate independently three times. Shown are representative photomicrographs of WT-NY72 and KO-NY72 cells at the time of quantification. Results are means ± SEM of data from three experiments. (B) Focus formation. WT-NY72 and KO-NY72 cells were plated at subconfluence in 60-mm dishes and cultured for 1 week at 37°C and 40°C. Foci were stained with Giemsa solution, and the number was determined. Means ± SEM of three independent experiments are shown.