FIG. 2.

Loss of SIRT1 in macrophages promotes NF-κB-dependent cytokine release and inhibits NF-κB-mediated apoptosis. (A) Elevated proinflammatory cytokine message levels in Mac-SIRT1 KO BMDMs. (B) Increased mRNA expression of NF-κB target genes in Mac-SIRT1 KO cells following TNF-α (10 ng/ml) treatment at the indicated time points. (C) Increased release of proinflammatory cytokines from Mac-SIRT1 KO BMDMs measured by ELISA following 12-h TNF-α treatment. (D) SIRT1 deficiency protects BMDMs from TNF-α-induced apoptosis. BMDMS from control and Mac-SIRT1 KO mice were treated with TNF-α and stained with propidium iodide. The data are represented as means ± SEM; n = 3; *, P < 0.05.