FIG. 6.

Microinjection does not affect general cellular health and allows visualization of the overlapping distribution of MeCP2 and H1⁰. (a) Fluorescence imaging indicates that microinjected TMR-H1⁰ (red) colocalizes with MeCP2-GFP (green), especially in PHC (merged image at right). Scale bar = 5 μm. (b) Images of H1⁰-GFP-expressing nuclei indicate that H1⁰-GFP (green) and microinjected TMR-MeCP2 (red) colocalize particularly well in PHC (merged image). Hoechst staining (cyan, far right) shows a very similar distribution. (c) Microinjected cells can be identified by the red fluorescence of Texas Red-dextran in the cytoplasm. Microinjection does not alter the nuclear morphology or the fluorescence of H1⁰-GFP (green). Scale bar = 20 μm.