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. 2010 Aug 16;30(20):4840–4850. doi: 10.1128/MCB.00453-10

FIG. 3.

FIG. 3.

Srs2 is required for checkpoint activation in CLUV-exposed rad18Δ cells. (A) CLUV-induced Rad53 phosphorylation. Protein extracts from cells exposed to CLUV for the indicated times were prepared and analyzed by 6% SDS-PAGE followed by Western blotting using an anti-Rad53 antibody. (B) Rad53 phosphorylation in CLUV-exposed PRR-deficient cells. Protein extracts from cells exposed to CLUV for 3 h in were prepared and analyzed by Western blotting with anti-Rad53 antibody.