FIG. 6.

Phosphorylation of Srs2 is required for HR-mediated recovery after release from CLUV exposure in rad18Δ cells. (A) The indicated strains were grown in the presence or absence of CLUV for 3 h, and the phosphorylation status of Srs2 and Rad53 was analyzed by immunoblotting. (B) Same experiment as in panel A, except that samples were subjected to FACS analysis. (C) Cell survival after CLUV irradiation was determined as for Fig. 1A. (D and E) Cells were treated with CLUV to activate the checkpoint and then removed from CLUV exposure to allow recovery. Aliquots were taken at the indicated times and processed by flow cytometry (D) or immunoblotting with anti-Rad53 (E). (F and G) rad18Δ and rad18Δ srs2-7AV mutants were analyzed for the presence of Rad52-YFP (F) or RPA-YFP (G) foci at the indicated time points after release from CLUV exposure. The error bars indicate the standard deviations.