FIG. 6.

p65 rescues TERT assembly mutants but not TERT activity mutants. (A) Linear diagram outlining the domains and motifs of TERT. The mutations studied are indicated below the motif in which they occur. (B) Telomerase assays for several of the mutants tested with wild-type TER in the absence and presence of p65. (C) SDS-PAGE analysis of a selection of telomerases containing the indicated TERT mutations assembled in the absence and presence of p65. The samples are shown in duplicate, and the ³⁵S-labeled TERT and coimmunoprecipitation of ³²P-labeled TER are indicated. (D) Quantitation of the telomerase activity and RNA pulldown of each mutant in the absence (white bars) and presence (gray bars) of p65. The top panel shows the relative telomerase activity for each mutant; error bars indicate SEM (n ≥ 3). The bottom panel shows relative RNA pulldown in the absence and presence of p65 for each mutant; error bars indicate SEM (n ≥ 3). Samples in both panels were normalized to wild-type TERT without p65.