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OPN mediates the support of LTC-ICs on MEFs. (A) The number of BM cells on Med1−/− MEFs in the presence of rmOPN recovers to the control level. (B) The number of CFCs on Med1−/− MEFs increases in the presence of rmOPN in a dose-dependent manner. (C) The number of CFCs on Med1+/+ MEFs is reduced in the presence of the anti-mOPN antibody. The values are the means ± SD of a representative experiment performed in triplicate (*, P < 0.05; **, P < 0.01).
