FIG. 2.

Receptor binding specificity of AdHu26. CHO-CAR and CHO-HVEM cells were infected with 10⁴ vps/cell of AdHu5GFP or AdHu26GFP. Cells were incubated for 24 h and evaluated microscopically (A). CHO-CAR or CHO-HVEM cells were incubated for 2 h with the Ad vectors expressing GFP, washed several times, and then incubated for an additional 22 h (2 h). Other cultures were incubated in the presence of AdGFP vectors for 24 h. Ad vectors were used at 10³ or 10⁴ vps per cell. Cells were trypsinized, stained with a live cell dye, and evaluated by flow cytometry. The histograms show the levels of GFP expression over the numbers of events (B). A 0.2% solution of red blood cells from a rhesus macaque was incubated with serial dilutions of AdC1, AdHu26, or saline without virus. The picture shows the original wells (C).