FIG. 3.

eIF2α and isoforms α, β, and γ of PP1c are coprecipitated with DP71L(s). Lysates from 293FT cells and 293 cells expressing the DP71L(s)-V5 fusion protein (DP-V5) were immunoprecipitated with an antibody against the V5 tag, and the pulled-down immunocomplexes were washed to remove nonspecifically bound proteins. Proteins that specifically bound to the beads (top row) and the input lysates (bottom row) were then analyzed by Western blotting (WB) using antibodies specific to the three isoforms of PP1c, eIF2α, and the V5 tag (indicated on the top of the panel).