FIG. 4.

Replication of recombinant rr-NCCR JCV in COS-7 cells. (A) Time course for prototypes CSF (patient 8), CSF-1 (patient 1), Mad-4, and urine (patient 1). DNase-protected JCV DNA was quantified by qPCR from cell supernatants collected at the indicated times after transfection. The data display the means from two independent experiments determined in triplicate. Error bars indicate the means ± standard deviations. (B) Immunofluorescence of JCV prototypes CSF (patient 8) (a), CSF-1 (patient 1) (b), Mad-4 (c), and urine (patient 1) (d) for VP1 (red) and agnoprotein (green) was performed at 6 dpt using monoclonal mouse anti-VP1 visualized with anti-mouse Cy3 and polyclonal rabbit anti-agno stained by anti-rabbit Cy2, respectively. Cell nuclei were detected by Hoechst 33342 dye (blue).