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. 2010 Aug 4;84(20):10448–10456. doi: 10.1128/JVI.00614-10

FIG. 5.

FIG. 5.

Modulation of NCCR-directed viral early and late gene expression in trans. (A) Effect of SV40 LTag on NCCR-mediated reporter gene expression. RFP-positive cells (red) were analyzed in CV-1 and COS-7 cells at 2 dpt. Early gene expression was normalized for at-NCCR-mediated expression in CV-1 cells. (B) Effect of HIV Tat on NCCR-directed reporter gene expression. At 2 dpt, RFP-positive (early) and GFP-positive (late) cells were analyzed after the transfection of pHRGNCCR reporter constructs into HeLa cells or HeLa SX cells constitutively expressing HIV Tat protein. The number of RFP-positive cells was set to 1 for at-NCCR-mediated reporter gene expression. The data display means from two independent experiments. Error bars indicate the means ± standard deviations.