FIG. 2.

UL97 phosphorylates Cdh1 at multiple sites in vitro. (A) In vitro kinase assays using purified Cdh1 and GST-UL97 were performed with [γ-³²P]ATP in the presence or absence of maribavir (MBV). ³²P incorporation was imaged on a phosphor screen, and the Coomassie-stained gel of the samples is shown below as a loading control. (B) The phosphopeptides identified by MS are listed with the phosphorylated amino acid shown by boldface with a lowercase p, and the corresponding position numbers are indicated. Cdk consensus sites and those conforming to the UL97 preferential sequence (P+5) are indicated.