FIG. 4.

Decreased APC4 and APC5 protein expression is observed during HCMV infection. (A) Levels of APC4, APC5, APC6, and APC3 protein expression were assessed by Western blot assay through an HCMV Towne (V) infection time course. GAPDH is shown as a loading control. (B) APC subunit expression was further examined in cells infected with ΔUL97 virus (Δ) and AD169 (V) by Western blot. Actin is shown as a loading control. (C) Total RNA was isolated from mock- or HCMV Towne-infected cells and analyzed for APC4, APC5, and G6PD expression by qRT-PCR. APC4 and APC5 values were normalized to the expression of G6PD as a control for input RNA. Values are expressed as the fold induction over the level at 0 h p.i.