FIG. 5.

APC4 and APC5 are degraded by the ubiquitin-proteasome pathway during HCMV infection. (A) HFFs infected with HCMV Towne (V) (MOI of 2) or mock-infected (M) were treated with proteasome inhibitor MG132 (2.5 μM, reversible) or Sal A (100 nM, irreversible) at 6 h p.i. Cells were harvested at 12 h p.i. or placed in fresh medium without drug and harvested at 24 h p.i. Samples were processed by Western blotting for protein expression. Lane numbers are shown below. (B) Mock-infected (M) or Towne-infected (V) cells were treated with Sal A (100 nM) or the E1 inhibitor PYR-41 (10 μM) at 6 h p.i. and harvested at 18 h p.i., and protein expression analyzed by Western blot assay.