FIG. 7.

Viral tegument and IE protein expression alone are insufficient to cause the loss of APC4 and APC5 protein expression. HFFs were treated with mock-infected (M), virus-infected (V), or UV-inactivated (UVi) tissue culture supernatants and harvested at 2, 4, 6, and 12 h p.i. To assess the requirement for IE expression, HFFs infected with HCMV Towne (MOI of 2) were treated with 100 μg/ml CHX from 2 to 12 h p.i. (+2), 4 to 12 h p.i. (+4), or 6 to 12 h p.i. (+6) to inhibit synthesis of early proteins. Samples were analyzed by Western blot assay. Short and long exposures of the UL44 blot are shown, and lane numbers are indicated below.