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16E5 does not disrupt cytoskeletal filament networks. Immunofluorescence microscopy showing actin, tubulin, vimentin, and cytokeratin filaments (green) in COS cells 24 h after transfection with AU1 epitope-tagged 16E5 or the empty pJS55 expression vector. Scale bar, 20 μm. The cells were colabeled with anti-AU1 antibody (red) to demonstrate 16E5 expression (insets).
