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Viral protein expression in infected human A549 cells. A549 cells either were infected with the recombinant VN/1203-WT, -ESEV, or -RSKV virus at an MOI of 5 or were mock treated (M). Cells were lysed at the indicated time points, and the lysates were analyzed for the viral proteins NS1, PB1, and NP by immunoblotting under identical conditions. Cellular actin was detected as a loading control.
