FIG. 6.

Determination of viral loads and the spread of VN/1203 viruses to different organs of mice. Groups of four 7-week-old female BALB/c mice were infected with 5 PFU of the VN/1203-WT, -ESEV, or -RSKV virus per mouse and were euthanized 12 h, 3 days (3 d), or 6 days postinfection. The indicated organs were prepared from infected mice at the indicated time points. Organs were homogenized, and cleared supernatants were analyzed by plaque assays on MDCKII cells. Virus titers were determined as PFU/g of tissue. Error bars indicate standard deviations.