TABLE 2.
MAb neutralization of different DENV-3 genotypesa
| MAb | Specificity | Domain location | EC50 for: |
|||
|---|---|---|---|---|---|---|
| gI 16652 | gII UNC3046 | gIII UNC3018 | gIV UNC3050 | |||
| DENV-3-E47 | Type | DIII | 110 | >5,000 | >5,000 | 4,600 |
| DENV-3-E48 | Type | DIII | 500 | >5,000 | >5,000 | >5,000 |
| DENV-3-E49 | Type | DIII | 8 | 4 | >20,000 | >20,000 |
| DENV-3-E50 | Type | DIII | 24 | >5,000 | >5,000 | >20,000 |
| DENV-3-E51 | Type | DIII | 20 | 25 | 514 | >5,000 |
| DENV-3-E52 | Type | E | 3,739 | ND | ND | ND |
| DENV-3-E53 | Type | DIII | 38 | 457 | >5,000 | >20,000 |
| DENV-3-E54 | Type | DIII | 9 | >5,000 | 1,982 | >20,000 |
| DENV-3-E56 | Type | DIII | 2 | >20,000 | 4,700 | >20,000 |
| DENV-3-E57 | Type | DIII | 6 | <1.0 | >5,000 | >20,000 |
| DENV-3-E58 | Type | DIII | 20 | >20,000 | >20,000 | >20,000 |
| DENV-3-E59 | Type | DIII | 42 | 3 | >5,000 | >20,000 |
| DENV-3-E60 | Type | DIII | 90 | 67 | >20,000 | 3,586 |
| DENV-3-E62 | Type | DIII | 9 | 162 | >5,000 | >5,000 |
| DENV-3-E63 | Type | DIII | 33 | ND | >20,000 | >20,000 |
| DENV-3-E64 | Type | DIII | 4 | >20,000 | >20,000 | >20,000 |
| DENV-3-E67 | Type | DIII | 5 | 383 | >20,000 | >20,000 |
| DENV-3-E55 | Subcomplex | DIII | >5,000 | >5,000 | 1,980 | >5,000 |
| DENV-3-E61 | Subcomplex | DIII | 281 | >5,000 | 99 | 3,084 |
a
The assay to determine focus reduction neutralization titers (FRNT) was performed on Vero cells after incubating 102 PFU of strains of the indicated DENV-3 genotype with increasing concentrations of purified MAbs. All EC50 values are indicated in ng/ml. Values are the averages of results from three to five independent experiments performed in duplicate. EC50 values were calculated by nonlinear-regression analysis. ND, not done.