TABLE 5.
Summary of MAb binding to DENV-3 DIII variants expressed on the surfaces of yeast cellsa
| MAb | % of control value for variant with: |
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L301T | S302N | S303A | V305G | L306G | K308E | E309K | K325E | D328G | D328N | A329G | A329T | A329V | P330G | G340N | A384Q | K386E | K386N | L385A | W389G | W391A | |
| DENV-3-E77 | 87.0 | 102.6 | 94.5 | 98.4 | 19.6 | 123.9 | 101.1 | 93.8 | 89.9 | 93.3 | 2.4 | 95.9 | 94.4 | 0.4 | 91.9 | 67.0 | 95.0 | 60.6 | 23.6 | 50.7 | 78.5 |
| DENV-3-E61 | 111.2 | 104.5 | 94.5 | 62.9 | 1.4 | 15.8 | 16.0 | 49.1 | 1.1 | 1.6 | 2.4 | 87.6 | 116.7 | 1.3 | 105.8 | 72.3 | 75.6 | 77.2 | 2.3 | 8.3 | 27.8 |
| DENV-3-E48 | 80.1 | 80.1 | 55.2 | 64.5 | 4.2 | 95.0 | 70.7 | 61.6 | 3.6 | 1.3 | 6.3 | 84.7 | 61.7 | 2.3 | 54.3 | 106.9 | 0.9 | 86.8 | 4.9 | 71.2 | 52.7 |
| DENV-3-E47 | 123.0 | 8.5 | 90.2 | 93.9 | 10.4 | 107.5 | 88.4 | 71.7 | 1.5 | 1.9 | 17.9 | 95.9 | 103.1 | 9.6 | 101.7 | 100.0 | 60.7 | 91.2 | 32.6 | 88.6 | 84.9 |
| DENV-3-E50 | 112.4 | 102.6 | 84.7 | 97.1 | 51.3 | 120.0 | 91.7 | 67.9 | 3.3 | 2.5 | 13.7 | 83.5 | 100.6 | 4.8 | 87.9 | 102.0 | 5.6 | 99.1 | 53.9 | 93.2 | 67.7 |
| DENV-3-E51 | 80.7 | 98.1 | 66.1 | 93.1 | 18.6 | 112.5 | 86.2 | 67.9 | 3.3 | 3.5 | 6.2 | 80.6 | 58.0 | 3.0 | 52.6 | 88.1 | 1.7 | 92.1 | 27.4 | 87.7 | 72.0 |
| DENV-3-E49 | 98.1 | 96.8 | 85.8 | 98.8 | 28.3 | 111.3 | 99.4 | 113.2 | 16.4 | 27.3 | 56.2 | 84.7 | 91.4 | 6.1 | 90.2 | 103.0 | 2.7 | 97.4 | 44.7 | 97.7 | 78.5 |
| DENV-3-E54 | 118.3 | 133.7 | 106.6 | 93.9 | 41.6 | 103.8 | 91.7 | 65.4 | 6.8 | 19.1 | 4.8 | 116.5 | 102.8 | 3.9 | 111.8 | 104.0 | 2.8 | 98.2 | 51.1 | 90.4 | 71.0 |
| DENV-3-E53 | 93.2 | 3.3 | 106.6 | 76.7 | 4.6 | 92.5 | 84.0 | 54.1 | 4.2 | 2.7 | 33.8 | 115.6 | 100.0 | 1.9 | 105.8 | 79.2 | 43.8 | 83.3 | 13.6 | 71.2 | 58.1 |
| DENV-3-E57 | 120.5 | 97.4 | 86.9 | 80.0 | 11.5 | 97.5 | 80.7 | 65.4 | 16.4 | 23.7 | 4.8 | 75.3 | 106.8 | 2.2 | 80.9 | 96.0 | 1.3 | 89.5 | 32.9 | 84.9 | 73.1 |
| DENV-3-E56 | 123.0 | 57.7 | 95.1 | 84.1 | 2.5 | 93.8 | 79.6 | 54.1 | 0.2 | 0.3 | 3.1 | 81.8 | 110.5 | 1.3 | 97.1 | 80.2 | 57.7 | 91.2 | 17.1 | 76.7 | 66.7 |
| DENV-3-E58 | 113.0 | 0.8 | 88.0 | 79.2 | 2.6 | 88.8 | 79.6 | 57.9 | 0.6 | 0.2 | 2.6 | 78.8 | 109.9 | 2.0 | 84.4 | 83.2 | 53.7 | 89.5 | 15.4 | 74.0 | 64.5 |
| DENV-3-E59 | 101.9 | 96.8 | 86.9 | 70.2 | 1.7 | 85.0 | 69.6 | 49.1 | 1.7 | 1.4 | 3.4 | 75.9 | 95.7 | 1.3 | 94.2 | 76.2 | 0.7 | 79.8 | 5.1 | 67.6 | 60.2 |
| DENV-3-E60 | 100.0 | 92.3 | 76.0 | 92.2 | 17.6 | 103.8 | 82.9 | 55.3 | 15.1 | 18.6 | 10.3 | 74.1 | 86.4 | 1.7 | 78.6 | 86.1 | 2.6 | 85.1 | 36.5 | 86.8 | 73.1 |
| DENV-3-E62 | 118.0 | 92.9 | 95.1 | 75.1 | 22.1 | 91.3 | 81.8 | 60.4 | 18.7 | 23.7 | 4.8 | 78.8 | 96.3 | 3.0 | 85.5 | 87.1 | 2.3 | 88.6 | 31.1 | 84.0 | 66.7 |
| DENV-3-E63 | 105.0 | 94.2 | 90.2 | 84.1 | 10.5 | 86.3 | 65.2 | 49.1 | 19.3 | 25.3 | 4.9 | 72.9 | 110.5 | 1.3 | 91.9 | 93.1 | 1.1 | 83.3 | 22.8 | 76.7 | 55.9 |
| DENV-3-E64 | 114.3 | 0.9 | 86.9 | 50.6 | 1.2 | 80.0 | 50.8 | 32.7 | 3.7 | 0.5 | 2.4 | 70.6 | 74.7 | 1.0 | 69.4 | 75.2 | 1.0 | 82.5 | 3.7 | 59.4 | 61.3 |
| DENV-3-E67 | 99.4 | 84.0 | 84.2 | 97.1 | 6.0 | 95.0 | 87.3 | 52.8 | 1.2 | 3.7 | 2.6 | 74.7 | 92.6 | 1.4 | 78.6 | 91.1 | 0.4 | 88.6 | 35.6 | 79.5 | 67.7 |
| DENV-3-E1 | 100 | 115 | 111 | 104 | 104 | 102 | 106 | 115 | 115 | 94 | 102 | 69 | 105 | 120 | 0.5 | 106 | 118 | 93 | 111 | 98 | 111 |
| DENV-3-E84 | 89.4 | 93.6 | 94.5 | 24.8 | 0.1 | 83.4 | 39.6 | 56.8 | 0.1 | 0.1 | 0.2 | 90.6 | 80.2 | 0.1 | 0.1 | 57.1 | 75.2 | 41.6 | 0.1 | 0.1 | 10.1 |
| DENV-3-E97 | 92.5 | 103.8 | 101.6 | 35.5 | 0.4 | 96.9 | 50.5 | 77.8 | 0.2 | 0.3 | 0.4 | 99.4 | 95.1 | 0.4 | 0.2 | 66.0 | 85.1 | 59.7 | 0.2 | 5.0 | 52.7 |
| DENV-3-E98 | 105.6 | 99.3 | 107.6 | 58.1 | 0.4 | 79.8 | 71.4 | 67.9 | 7.6 | 0.4 | 10.3 | ND | ND | 13.4 | 102.4 | 85.7 | 94.1 | 77.9 | 0.4 | 37.0 | 78.5 |
| WNV E111 | 94 | 69 | 26 | 2 | 60 | 15 | 35 | 2 | 2 | 4 | 4 | 7 | 92 | 50 | 68.3 | 58 | 82 | 2 | 4 | 9 | 10 |
a
Staining of yeast cells that express DENV-3 16652 DIII with single point mutations. Yeast cells were stained with 20 μg/ml of MAb. Data represent the averages of results from four to six independent experiments. Data are normalized to the percentage of cells that stained positive for DENV-3-E8. Boldface indicates that the level of binding of a given MAb was from 0 to 25% of the control value, and underlining indicates that the level of binding was from 26 to 50% of the control value.