FIG. 1.

Primary human MEC express HIV-1 receptors, coreceptors, and an alternate receptor. Confocal microscopy images of MEC stained for HIV receptors (left) and the same cells imaged by DIC (right). Nuclei are stained blue with DAPI. An immunomagnetic bead can be observed in the DIC image shown in panel D. (A) GalCer expression (yellow). (C) CCR5 expression (green). (E) CXCR4 expression (red). (G) CD4 expression (pink). Z-stack imaging confirmed the presence of receptors within the cytoplasm and/or nucleus as shown. Similar results were obtained in two additional subjects, with the exception of CD4 expression. CD4 mRNA was confirmed in three subjects by use of real-time PCR.