FIG. 2.
Primary human MEC do not support HIV reverse transcription or integration of the CCR5-, CXCR4-, or dual-tropic strains tested. PCR results for HIV-1 reverse transcription and integration of CCR5-tropic HIV-1BaL, CXCR4-tropic HIV-1HC4, and dual-tropic HIV-1C7/86 in both primary human MEC and PBMC controls. The bottom row shows PCR results for human beta-actin genomic DNA. Lanes 1 to 4, MEC; lanes 5 to 8, PBMCs; lane 9, negative control (no DNA). Experiments were performed in triplicate under each condition. Similar results were obtained with MEC from three additional subjects.