FIG. 2.

LANA augments the proliferative capacity of B cells in culture. (A to D) Examples of flow cytometric analysis of purified cultured B cells labeled with CFSE after 4-day stimulation with LPS (gray density) or nonstimulation control (open diagram). Stimulation is measured by the fraction of cells that lose fluorescent intensity. The horizontal line (and number) indicates the gate that was used for quantification. The CFSE fluorescence intensity is shown on the horizontal axis, and the number of cells as a percentage of the maximum is shown on the vertical axis. Results with B cells from LANA transgenic mice are shown in panels C and D, and results from wild-type littermate control animals are shown in panels A and B. Animals in panels B and D were in vivo stimulated by NP-KLH immunization prior to harvest. (E) Box-and-whisker plot of the results from multiple animals. A dot indicates the median, a box indicates the interquartile range (IQR), bars indicate the range, and open dots denote outliers (>1.5 × IQR). The three asterisks indicate a significant difference between groups of six LANA and six wild-type mice at P ≤ 0.005 as determined by using the Wilcoxon rank sum test.