FIG. 3.

Structure and localization of PML isoforms. (A) Schematic representation of the domain structures of PML isoforms. All PML isoforms share an N terminus but differ in their C termini due to the alternative splicing of exons 7 to 9; protein domains of all isoforms include the RING finger (R), the B1 and B2 boxes, the coiled-coil (CC) motif, the nuclear localization domain (NLS), and three SUMOylation sites. (B) PML isoform localization. U373MG cells expressing PMLI, PMLII, PMLIII, PMLVI, PMLV, or PMLVIIb were analyzed by immunofluorescence staining with anti-PML antibodies. The scale bars correspond to 20 μm.